Observation should take place with a total magnification of 100 – 200x and with phase contrast, because most cells are difficult to observe in normal bright field illumination. Since the cells are attached to the bottom of the dish, they can be viewed easily from this perspective. The optics of an inverted microscope are located below the specimen. After this, a closer check of the general cellular morphology and growth patterns should be examined using an inverted microscope. It is important to examine the cell culture every day and immediately prior to subculturing to monitor cell health, check for contamination and determine when to split the cells.Ī first examination of the culture for fungal contamination, turbidity and particles in the medium as well as unexpected pH shifts, indicated by color change of the medium, can be done at the macroscopic level, by eye.
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